Current Issue : October-December Volume : 2011 Issue Number : 4 Articles : 5 Articles
A simple, sensitive, accurate, precise, reproducible and cost effective UV spectroscopic method has been developed for the estimation of Diacerein in bulk form. Diacerein shows maximum absorption at 258 nm with molar absorptivity of 2.9682 × 104 l/mol.cm. Beer’s law was obeyed in the concentration range of 1-25 µg/ml. The method was validated for linearity, precision, accuracy, sensitivity and specificity. The data obtained was treated with the statistical approach. The proposed method was not only found to be accurate and precise for estimation of Diacerein in bulk drug substance. During method development priority was given to using environment-friendly chemicals....
Embelia ribes fruits, which are commonly known as Vidanga, have long history of use in ayurvedic system of medicine in various forms like asava, aristha, lauha, and taila. Vidanga Churna is an ayurvedic formulation of Embelia ribes fruits and the active component in vidanga is embelin. One in-house batch of vidanga churna was prepared in laboratory and standardized according to WHO guidelines. Three different marketed formulations were procured from Ayurvedic medicine shop. Embelin was isolated from the in house churna and its identity was confirmed by physical data and spectroscopic studies. Different tests like determination of foreign matter, macroscopic and microscopic examination, determination of ash value, extractable matter, swelling index, foaming index and microorganisms were performed as per WHO guidelines and limits for the same were optimized. A UV spectrophotometric method was developed for the estimation of embelin in laboratory and marketed formulations. The content of embelin in raw material was found to be 2.2 % w/w. The developed method has been validated as per ICH guidelines. Developed spectrophotometric method shows good regression (0.9982) and the recovery of the embelin is in the range of 98.64-102.38 %. The limit of detection and limit of quantitation are found to be 0.05 µg/ml and 0.157 µg/ml, respectively. The results of the study revealed that the developed UV spectrophotometric method is simple, precise, and reliable for quantitative analysis, moreover, for the quality evaluation and control of vidanga....
The fixed oil of the seed of Buchanania lanzan spreng, belonging Family Anacardiacea was analyzed using GC-MS. Nine saturated fatty acids compounds from B. lanzan spreng were characterized. Methyl ester Pentadeconic acid (55.60%), 11-Octadecanoic acid (10.40%), Hexadecanoic acid (9.90%), Docosanoic acid (7.24%), Eicosanoic acid (5.81%), Cyclopropanoic acid (3.78%), 9-Octadecenoic acid (2.91%), Oleic acid (1.80%) and Henicosanoic acid (1.34%). The results shows that the content of fatty acids was abundant in Buchanania lanzan spreng, and it had a great range of potential utilities and a prospect of development in foods medical and health care’s....
This paper describes the analytical method suitable for validation of Pioglitazone by UV Spectrophotometric method. The method utilized UV spectroscopy (Shimadzu, model 1700).The solvent system was consists of Methanol at wave length (λ max) 226 nm. Validation experiments were performed to demonstrate System suitability, Specificity, Precision, Linearity, Accuracy Interday assay, intraday assay, robustness, ruggedness, LOD, &LOQ. The method was linear over the concentration range of 5-60 µg/ml. The method was showed good recoveries (99.49- 100.09%) and the recovery studies were carried out by adding different amounts (80%, 100% & 120%) of bulk samples of Pioglitazone. The Proposed method was simple, sensitive &reliable with good Precise, Accurate, and Reproducible and rapid for the determination of Pioglitazone. While estimating the commercial formulation without interference of excipients & other additives .hence this method can be used for routine determination of Pioglitazone in bulk and their pharmaceutical dosage forms....
In present study, a successful attempt has been made to develop a simple method for the simultaneous determination of Paracetamol and Aceclofenac using UV spectrophotometric technique, the Proposed method was found to be simple, precise, and accurate and can be successfully applied for routine quality control analysis and simultaneous determination of Paracetamol and Aceclofenac in combined bulk form. The method permits the determination of Paracetamol & Aceclofenac over a concentration range of 4µg/ml to 32µg/ml. All the calibration curve shows a linear relation between the absorbance and concentration with correlation coefficient 0.999. Multicomponent method of analysis was validated by analyzing the laboratory prepared samples. The % mean results were found to be 99.634 of Paracetamol & 100.71 of Aceclofenac respectively. Similarly the standard deviation of Paracetamol & Aceclofenac was found to be 0.427 & 1.470 respectively which indicate that the method has accurate, precise & sensitivity....
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